pcep4 vector Search Results


pcep4-p53 175  (Boehringer Mannheim)
90
Boehringer Mannheim pcep4-p53 175
<t>p53</t> mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either <t>pCEP4-p53143</t> (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).
Pcep4 P53 175, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcep4-p53 175/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
pcep4-p53 175 - by Bioz Stars, 2026-03
90/100 stars
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pcep4 vector  (Fisher Scientific)
90
Fisher Scientific pcep4 vector
<t>p53</t> mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either <t>pCEP4-p53143</t> (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).
Pcep4 Vector, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcep4 vector/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
pcep4 vector - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pcep4 vector  (Crucell Inc)
90
Crucell Inc pcep4 vector
<t>p53</t> mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either <t>pCEP4-p53143</t> (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).
Pcep4 Vector, supplied by Crucell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcep4 vector/product/Crucell Inc
Average 90 stars, based on 1 article reviews
pcep4 vector - by Bioz Stars, 2026-03
90/100 stars
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pcep4 expression plasmids for flag-tagged pit-1  (Kodak)
90
Kodak pcep4 expression plasmids for flag-tagged pit-1
<t>p53</t> mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either <t>pCEP4-p53143</t> (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).
Pcep4 Expression Plasmids For Flag Tagged Pit 1, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcep4 expression plasmids for flag-tagged pit-1/product/Kodak
Average 90 stars, based on 1 article reviews
pcep4 expression plasmids for flag-tagged pit-1 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


p53 mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either pCEP4-p53143 (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).

Journal:

Article Title: p53 associates with and targets ?Np63 into a protein degradation pathway

doi:

Figure Lengend Snippet: p53 mutants that bind to ΔNp63α mediate its degradation. (A) p53143 or p53273 mediates degradation of ΔNp63α in transfected cells. 022 cells were transfected with either pCEP4-p53143 (1 μg, 5 μg) or pCEP4-p53273 (1 μg, 5 μg). Total lysates were resolved by SDS/PAGE and probed with antibodies to p53, ΔNp63, or β-actin. (B) The half-life of p40 in the presence of overexpressed p53WT or p53175. Cells infected with adenoviruses for 18 h were labeled with 200 μCi/ml [35S]methionine for 1 h. Labeled cells were then maintained in nonradioactive medium for 0 min, 60 min, 90 min, 120 min, 3 h, or 6 h before collecting (data for 3 and 6 h not shown). Total lysates were precipitated with antibody to ΔNp63 and resolved by SDS/PAGE followed by autoradiography. The immunoprecipitates were normalized by trichloroacetic acid-precipitable radioactive material, and equal amounts of total radioactive lysates (dpm/mg of total protein) were used for experiments. p53WT overexpression decreases the p40 half-life (by ≈80%).

Article Snippet: The mammalian expression cassettes pCEP4-p53 WT , pCEP4-p53 143 , pCEP4-p53 175 , pCEP4-p53 248 , pCEP4-p53 273 , pCEP4-ΔNp63α, and pCEP4 (empty vector) were used for transient transfection of SaOs2 cells with 5 μg of DNA and 10 μl of FuGene-6 (Boehringer Mannheim).

Techniques: Transfection, SDS Page, Infection, Labeling, Autoradiography, Over Expression